Data Analysis K-S and S-W tests?

Hello, I have conducted analysis where the K-W and S-W have come out as p

25 February 2021 9,648 3 View

Simple clearing method for observation of hand-sectioned wheat leaves?

I am using a toluidine blue O preparation for staining different parts of wheat plants to observe a fungus growing in them. This works really well for lighter tissues of the plant! However, the...

27 October 2020 9,159 1 View

How to Perform Feature Selection with Multilevel Encoded Categorical Variables?

I'm attempting to do feature selection on the variables on my dataset, but unfortunately the overwhelming majority of them are categorical. Currently they've all been encoded into binary...

29 July 2020 7,581 3 View

3D Slicer to ANSYS or FEBio?

Hi all, Does anyone have a workflow for preparing volumetric meshes in 3D Slicer and importing them into finite element software such as ANSYS or FEBio? Been trying so many approaches but they...

26 July 2020 7,012 3 View

How do I report Multiple Regression Analysis with imputed values SPSS?

I'm conducting multiple regression in SPSS with data where I've replaced missing values using Multiple Imputation. The output gives the pooled values in the coefficients table, but for the model...

10 June 2020 2,069 2 View

Is there a way to set random initial values for occupancy models in R using the RPresence package? Or any documentation file for RPresence?

I am trying to set random initial values for some occupancy models in R, in response to getting some convergence warnings. I am using the RPresence package but cannot find any documentation files...

09 February 2020 3,057 2 View

Removing vitreous from baby rat eyes?

I have been trying to remove the vitreous humour from baby rat eyes (post natal day 15) and have been still having a lot of interference in my IHC staining. Does anyone have a good protocol to...

20 January 2020 4,634 1 View

Colony Formation assay vs Growth assay - do they report different things?

I'm looking at the response of an established cell line to addition of a drug in terms of growth inhibition. Whilst I see a slight growth inhibition when seeding cells 10-25% confluent (I have...

08 December 2019 319 1 View

GDNA stability at 55 degrees?

I was extracting genomic DNA from Streptomyces the previous day and left my gDNA in the oven (55 degrees Celsius) to dry at the very last step. I had to rush off for a seminar and completely...

11 September 2019 9,441 2 View

How does freezing antibiotics result in loss of activity?

I remember my mentor saying before that she decided to refrigerate her gentamycin stock solution instead of freezing it since that was the recommended storage condition. Now, I'm dealing with...

04 August 2019 5,766 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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22 February 2021 9,932 11 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View