My goal is to visualize neuronal glial reactions. If I have brains that are Golgi stained, is it possible to double label them with Iba1 for visualization in a light microscope?
I am aware this can be done through EM or sophisticated fluorescence/confocal strategies but my query roots from having to do things in tight budgetary constraints.
Article Differential staining of glia and neurons by modified Golgi-Cox method
You could also try improving it by using a 4% Paraformaldehyde and 0.5% Glutaraldehyde, as glutaraldehyde increases histology resolution in some reactions. Although it can also inhibit some enzyme activity depending on the enzyme.
What particular region are you studying?
Most colour systems do not coincide with the black colour of Golgi or are visible with Golgi stained elements. Only fluorescence staining seems possible. Marani
it is highly unlikely that this can be done. golgi staining requires harsh treatments which may disrupt any other antigen. plus, the black silver precipitate absorbs a lot of UV light, so fluorescence is most likely out of question.
Zuhayr Khan , Pre Frontal Cortex, Hippocampus and Amygdala. The big three in chronic stress.
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