I've searched the literature and there seems to be many options. For example, either neutral red or crystal violet are used to stain to help visualize plaques. Why and when is either used? Also, sometimes a fixative is used. Why or why not would I do that?
for me, I do not use both for influenza virus, I can count the plaques visually or under microscope ..... do not think about this too much, they are just for visualization, think about your calculations and technique especially while mixing and pipetting to make different dilutions ...
Hi Alicia,
You can do plaque assay by using Crystal violet dye on Vero cells. But i strongly recommend you to switch over to Immuno-Plaque assay using serotype-specific Dengue antibodies, which is a very good and precise method suggested for Dengue virus quantification. I do this method and found it is a terrific technique.
Good Luck.
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