Does anyone has an idea about in detail procedures of indirect ELISA validation?

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MD MOE RMSD

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fatal error

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fatal error

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fatal error

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fatal error

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Shahzad Ali

this may work for you

Tiziana Di Febo

I do validation of ELISA tests (indirect and competitive) following the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial animals, Chapter 1.1.5. "Principles and methods of validation of diagnostic assays for infectious diseases". After analysis of positive and negative sera, cut-off, sensitivity and specificity can be calculated by ROC curve analysis.