I know that there are many commercial available kits from FFPE, but I would like to extract RNA from a cardiac tissue sample preserved only in formalin.
The RNA extraction process per se will perform similarly for either FFPE or Fomalin-only fixed, therefore the use of the same FFPE kit is fine. You only need to skip the deparafinisation step. In fact, I use RNeasy FFPE Kit which works well for purifying total RNA from formalin-fixed and/or paraffin-embedded tissue sections (and for Formalin-fixed I skip the Deparafinisation solution). Good luck.
Thank you. I have been looking for this solution and it perfectly explains what i thought. But i would like to know whether this affects the quality o RNA in any way.