I have just started to work with mice hippocampal primary cultures. Can anyone suggest me both EGF and bFGF specific for mice cell cultures?
Thanks,
I am looking for a protocol that would allow me to isolate live cells from mice adult hippocampus (7-8 weeks old). I am mostly interested on neurons and microglia. At the moment I have tried this...
03 April 2019 3,232 3 View
I am looking for the best way to analyse phospholipids. I am not interested on fatty acids but total phospholipids such as phosphatidylethanolamine, sphingomyelin, phosphatidylcholine,...
07 August 2016 514 3 View
I work with rat cortical primary cultures and looking for the best ELISA kit for the detection of BDNF protein levels. I am also wondering if detection is possible either from supernatant or from...
03 April 2015 573 3 View
Hi all, I am wondering if it is possible to detect levels of cytokines from plasma of healthy rats either immediately after an acute stress or after a week from the stress. Thanks, Matteo
06 July 2014 7,291 12 View
I am having some difficulties on finding papers related to the effect of phospholipids, such as phosphatidylserine and phosphatidylethanolamine, on brain primary cultures, in terms of...
03 April 2014 4,164 1 View
I work with cortical primary culture from a rat and I need to do an investigation regarding phosphorylated glucocorticoid receptors by immunostaining. Unfortunately, I have found Abs that suit for...
02 March 2014 3,896 2 View
I work with rat cortical primary culture and need to detect eventual astrocyte proliferation using western blot technique
02 March 2014 7,818 5 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View