Matteo Pusceddu

8 Questions 9 Answers 0 Followers

Questions related from Matteo Pusceddu

How to isolate live cells from mice adult hippocampus?

I am looking for a protocol that would allow me to isolate live cells from mice adult hippocampus (7-8 weeks old). I am mostly interested on neurons and microglia. At the moment I have tried this...

04 April 2019 3,232 3 View

Does anyone can suggest me what EGF and bFGF should I use for MICE hippocampal primary cultures?

I have just started to work with mice hippocampal primary cultures. Can anyone suggest me both EGF and bFGF specific for mice cell cultures? Thanks,

10 October 2017 7,160 0 View

How to analyse phospholipids levels?

I am looking for the best way to analyse phospholipids. I am not interested on fatty acids but total phospholipids such as phosphatidylethanolamine, sphingomyelin, phosphatidylcholine,...

08 August 2016 515 3 View

Can anybody suggest a good ELISA kit for BDNF protein levels from rat cell cultures, please?

I work with rat cortical primary cultures and looking for the best ELISA kit for the detection of BDNF protein levels. I am also wondering if detection is possible either from supernatant or from...

04 April 2015 573 3 View

Can you detect cytokines levels from plasma of healthy rats?

Hi all, I am wondering if it is possible to detect levels of cytokines from plasma of healthy rats either immediately after an acute stress or after a week from the stress. Thanks, Matteo

07 July 2014 7,291 12 View

Can anybody suggest good papers related to the effect of phospholipids treatment on brain primary culture cells?

I am having some difficulties on finding papers related to the effect of phospholipids, such as phosphatidylserine and phosphatidylethanolamine, on brain primary cultures, in terms of...

04 April 2014 4,164 1 View

Any suggestions for phospho-GR RAT Ab for immunostaining?

I work with cortical primary culture from a rat and I need to do an investigation regarding phosphorylated glucocorticoid receptors by immunostaining. Unfortunately, I have found Abs that suit for...

03 March 2014 3,896 2 View

Does anybody know specific antibodies for astrocyte proliferation detection using western blot in rat brain primary cultures?

I work with rat cortical primary culture and need to detect eventual astrocyte proliferation using western blot technique

03 March 2014 7,818 5 View