Does anyone analyse Relative Real-time PCR data with REST-MCS 9 august 2006 tool?

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Vasileios Kordinas

I have used REST 2009 tool and this was not an issue. If you have timecheck the following links

http://rest.gene-quantification.info/

http://www.gene-quantification.de/rest-2009.html

http://www.gene-quantification.de/rest-xl.html

Ivan V Bogdanov

I have to use REST-MCS, because it is support multiple experimental conditions: one reference condition and up to 6 different treatments.

Chan Nguyen

REST is actually a fomulared excel file. The data from A13:A20 is taken from the range A52:A59, where you can add the serial dilution for the very first reference gene. The serial dilution of DNA and efficiency of each primer pair are obtained by the validation run.

By the way you can change the number of reference genes, target genes, and conditions by changing the names in the "introduction" tab. I did 3 ref genes, 3 target genes, and 4 conditions. All good.