Ivan V Bogdanov

2 Questions 12 Answers 0 Followers

Questions related from Ivan V Bogdanov

Can amplification rate be more than 2 in gene expression experiments?

The same primer pairs with cDNAs, reverse-transcribed from different plant tissues, give different amplification rates which are almost always above 2. Can these data be used or I should optimize...

03 March 2016 3,141 7 View

Does anyone analyse Relative Real-time PCR data with REST-MCS 9 august 2006 tool?

Hi! I have a quick question about amplification efficiency calculation. There is a strange range for amount of cDNA input (ng) in this tool: 1, 0.1, 0.01, then 5, 2, 1 again, 0.5, 0 (see PrtScr in...

03 March 2016 6,120 3 View