MDCK cells are known to be trypsin resistant, and very long to detach from the dish. I read in some protocols that a trypsin (10x) could be used and would be more efficient. I think it could be a more aggresive, but a quicker treatment. It's a balance. Does anyone already tried a more concentrated trypsin for those cells?
Ilya Korotetskiy
Hello,
You are right about trypsin resistance. You can try wash the monolayer with Hanks solution or PBS before adding trypsin. Moreover, try to incubate at 37 °С and 5 % СО2. After 5-7 min our cells are detached from the flask.
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