We perfomed a corticosterone ELISA from ENZO and our data are misleading. The sample we analysed contain only low concentrations but we detected an OD that hypothesized high values. I think there might be an crossreaction with other molecules. Do anyone has an Idea what to do?
The ENZO kit is a competitive one, which means that high OD shows low sample corticosterone. If i read well. (ADI-900-097).
yes that is right and this is my problem. We have low concentrations and an low OD.
ANd I have no idea why.
Did anybody else used this ELISA before and have similar problems?
Agree with Attila. OD is inversely proportional to cortisone concentration.
one is the whole antibody system can be wrong. other the plate-bounded conjugate went wrong. sign ENZO what is your issue. did your control is OK on that plate? the negative sample should show a high value, i think.
Manuella, have you performed the assay with known concentrations samples that should be in the ENZO kit? Is it working properly?
yes we performed the assay with known concentrations, negative control, blank and everything worked fine. Also with our samples wih 1:10 dilution the OD was within the range. But at 1:100 dilution the OD was outside the standard curve and low. And there is my problem! These OD have to be high because of the comparative ELISA system.
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