How surface tension of solvents affect crystallization of nanoparticles in a sol-gel method?

In the synthesis of metal oxide nanoparticles by sol-gel method, how surface tension of solvents affect crystal growth?

06 January 2021 3,687 3 View

For RNA seq study and data analysis, How many tests (e.g. duplicate or triplicate) should be considered for each sample?

As we are doing RNA seq for some cell lines before and after treatment, how many tests should we do for each sample to validate data statistically?

04 January 2021 6,436 2 View

I need to wash carboxy methyl chitosan with 70%ethanol until neutral pH obtained, how can I do this task without consuming to much ethanol?

in the last step of synthesizing carboxy methyl chitosan from chitosan we need to wash it with 70%ethanol until neutral pH obtained, to achieve this goal we need to consume lots of ethanol, I am...

21 September 2020 2,060 2 View

I need to get some references on the topic of thermal conductivity of building materials. Who can help me ??

scientific researches Master Theses PhD Any previous studies benefit me in my research ... Thank you

31 May 2020 1,031 14 View

Whats the coordinate of atoms in a crystal with cubic structure that atomic positions for them are same, like 4a(0,0,0)?

Whats the coordinate of atoms in a crystal with cubic structure that atomic positions for them are same, like 4a(0,0,0)?

24 February 2020 6,351 3 View

I need a single-phase voltage-dependent variable load in MATLA/Simulink. How can I do that?

I need a variable load as I can control its power or its impedance via changing its voltage. Not a variable current controlled loads. I would be grateful if somebody could help me.

14 July 2017 9,840 3 View

Connection Spring Model in SMF's with RBS section ?

Dear Friends, Does anyone know how to model connection spring in steel moment frames, I mean the spring in the face of column in steel moment frames with RBS section ?

11 January 2017 9,786 3 View

Is it possible to compute ion conductivity using existing Gromacs tools? How might I calculate ionic conductance through bilayer in gromacs?

ionic current, gromacs

26 May 2015 2,572 1 View

How can I achieve the second-order optical gain in quantum dots?

15 May 2015 7,785 3 View

Is it safe to use a regular tube furnace along with working tube and gas tight end seals to do reduction treatments under a pure hydrogen atmosphere?

I would appreciate any details that I should consider before starting my treatments.

06 May 2014 8,884 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View