As we are doing RNA seq for some cell lines before and after treatment, how many tests should we do for each sample to validate data statistically?
triplicate is required.
Definitely at least 3. Less will result in a large number of false-positive reads during your differential expression analysis.
Dear scholars, I was wondering if there are any scientific methods to train a neural network or ANFIS when one of the input variables is stochastic (random) and more importantly non-measurable?...
17 February 2021 1,909 3 View
Dear scholars, I was wondering how can I generate MATLAB code of an ANFIS (FIS) model? I need to integrate my ANFIS (FIS)/Fuzzy logic system into another MATLAB code to render optimizations. Do...
04 February 2021 6,098 4 View
In the synthesis of metal oxide nanoparticles by sol-gel method, how surface tension of solvents affect crystal growth?
06 January 2021 3,687 3 View
In arid regions, what environmental factors have a greater effect on the establishment and germination of rangeland plants? Describe the effectiveness of the priority
16 November 2020 3,642 9 View
Why the number of secondary depressions on existing chromosomes varies?
15 November 2020 9,614 3 View
in the last step of synthesizing carboxy methyl chitosan from chitosan we need to wash it with 70%ethanol until neutral pH obtained, to achieve this goal we need to consume lots of ethanol, I am...
21 September 2020 2,060 2 View
Dear scholars, Do you have any suggestions for a concise and useful book with examples covering continuous numerical value predictions? Suppose that, we need to use regression techniques for...
05 August 2020 8,422 3 View
What does it mean?
03 August 2020 734 2 View
scientific researches Master Theses PhD Any previous studies benefit me in my research ... Thank you
31 May 2020 1,031 14 View
any body knows a relation between RSSI and CM? Basically, RSSI is used for distance estimation between the RFID tag and RFID reader antenna. but recently RSSI has been used for other findings as...
26 April 2020 6,429 3 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
02 March 2021 3,060 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View