For RNA seq study and data analysis, How many tests (e.g. duplicate or triplicate) should be considered for each sample?

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How to calculate the mode volume in metal nanoparticles?

Can the mode volume in smaller metal nanoparticles be larger than the nanoparticle volume?

18 July 2024 4,859 4 View

How much variable should be included for master thesis?

Currently working on my master's thesis proposal, which involves exploring the interplay among 7 variables. This has led to the formulation of 13 research hypotheses. I'm curious to hear thoughts...

08 March 2024 4,344 2 View

How can I know that the energy is transferred from plasmon to exciton or from exciton to plasmon?

I have a plexciton hybrid particle which consists of plasmon and exciton. How can I know that the energy is transferred from plasmon to exciton or from exciton to plasmon? I also know that this...

15 January 2024 1,442 3 View

Seeking Advice on Seal Selection for High-Pressure, High-Temperature Reactors?

Hi 👋 I hope this message finds you well. I am currently collecting data on the design of a reactor system operating at 200 bar pressure and 300 degrees Celsius temperature, with a reactor volume...

28 December 2023 6,795 1 View

What is the Q-band in absorbance spectra?

In some absorbance spectra in the articles, a region called the Q band can be seen. Where did this name Q come from and what is the physical interpretation of these absorption peaks?

27 November 2023 7,332 5 View

What is plasmon-plasmon coupling?

As we can define a coupling strength between plasmon and exciton. Can we define a coupling strength between the plasmons of one material and another material?If we know the location of the plasmon...

10 July 2023 3,916 4 View

What is exciton-exciton coupling?

As we can define a coupling strength between plasmon and exciton. Can we define a coupling strength between the excitons of one material and another material?If we know the location of the exciton...

10 July 2023 3,899 4 View

Can the "Rchoice" package compute marginal effects for a correlated random parameters ordered probit model?

04 May 2023 3,914 2 View

Why don't black soldier fly eggs hatch?

The temperature of keeping eggs is 27 degrees Celsius and the humidity of the environment is 70%.

19 April 2023 9,205 1 View

What criteria are there to consider a plant species as an invasive species in rangeland ecosystems?

What criteria are there to consider a plant species as an invasive species in rangeland ecosystems? Are there any standard protocol to evaluate the invasionsess situation of species?

27 February 2023 3,979 7 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View