Dear all,
I`m working with the cuprizone mouse-model and I need to know how cuprizone can be inactivated. I checked the internet several times. Maybe somebody of you can give me a hint....
Thank you!
Dear Sandra,
I only can tell you that cuprizone is sensitive to light and high temperatures and it is degraded under these conditions, but I can't give you a good reference.
Good luck!
Dear Sandra,
I would like to know something about your aim, because could be easiest to answer.
take care
Dear Giovanni,
I want to know how cuprizone can be inactivated in order to proof and improve the correct handling with cuprizone. I want to performe in vitro expiriments with oligodendrocytes (and check the changes in differentiation) and in vivo studies, to see if mice still get sick from cuprizone after it was heated.
Dear Sandra,
I am working with cuprizone in culture and I can tell you that it is not deactivated at 37°C (which is the temperature I used to dissolve it in a mixture of H2O and EtOH).
I have attached you the paper where I found the protocol I am currently using.
http://www.ncbi.nlm.nih.gov/pubmed/?term=Cuprizone+[Bis%28Cyclohexylidenehydrazide%29]+is+Selectively+Toxic+for+Mature+Oligodendrocytes
Hi Sandra,
Cuprizone is a very confusing chemical, I have not come across any thing that can inactivate it. I think Thomas from Hanover Medical School might know as he is working this field for long time. However, heat may inactivate it but there are two research papers which give confusing results one said cuprizone function suppress if you heat it up (120 degrees) and another one said it has no effect.
Both papers are attached,
Have a good luck!
Monokesh
Article Cuprizone-Containing Pellets Are Less Potent to Induce Consi...
Article Investigation of Cuprizone Inactivation by Temperature
- Badges
- Science topic
- Similar topics
- Chemistry
- Pharmacology
- Pharmaceuticals
- Drugs