Ana Bernal-Chico

4 Questions 7 Answers 0 Followers

Questions related from Ana Bernal-Chico

Sudan black staining and TESPA coating, protocols?

Hi everyone: I would like to try a Sudan Black myelin staining and I red that it is recommended to use TESPA-coated glass slides. Could anyone send me a protocol? Are there any "tricks" that I...

19 June 2025 9,143 2 View

Can anyone recommend a good antibody to detect CCL2 in animal (mouse) tissue (brain)?

I want to label CCL2 in the brain parenchyma of mice after an inflammatory insult. I have tried 2 antobodies, one of them worked in vitro, but I am not able to detect anything in brain slices. Can...

14 November 2022 4,885 0 View

Can anyone recommend dosage in use of Lambda Protein Phosphatase?

Hello everybody. I want to desphosphorylate a protein extract from tissue homogenate with Lamba Protein Phosphatase. In the data sheet doesn't specify which quantity or how many units of...

28 January 2016 3,837 2 View

What are some protein deglycosylation protocols for immunohistochemistry?

I have found some protocols to deglycosylate proteins in a tissue extract to perform western blotting but anybody knows if there are any available tools to deglycosylate wholes tissue slice to...

01 October 2015 9,334 8 View