Does anybody know what the electrical parameters of electroporation of human embryonic stem cells are?

21 February 2013 0 467 Report

We have a BTX electro square Porator (ECM 830) and we want to transfect some plasmid or TALEN's in hESC.

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Publishing a checklist?

Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...

01 August 2024 6,134 2 View

What are the most adequate analytical techniques for food contaminants analysis?

Good morning, Because food contaminants can be of different natures, is there a compendium of analytical techniques, standardized preferably, that can be consulted? Related to that question,...

15 February 2024 6,706 2 View

Potential for Y-H?

Does anyone know where to find the potential Y-H to perform MD simulations? I could not find it in the literature or in repositories. Many thanks in advance.

13 November 2023 7,268 0 View

Does genre imbalance impact Logistic Regression and Classification Models?

Seeking insights from the research community: Does the imbalance of textual genres within corpora, when used as an explanatory variable rather than the response variable, affect the performance of...

22 July 2023 1,790 3 View

How to disolve GSK126 in DMSO And Captisol for an in vivo use in mice?

Hello Dear Community Does somebody has a protocol to dissolve the GSK126 at a concentration of 12 to 15 mg/mL in DMSO and Captisol? Thank you by advance for your help Christophe

05 June 2023 6,171 2 View

How can technology (or innovation) negatively affect economic growth (or development), especially in countries with low technology levels?

Hello to all, I'm actually interested in the effects of technology on the level of economic development as measured by real GDP per capita. My results robustly indicate that in countries with low...

05 April 2023 4,498 11 View

What are the specific aspects of enterprise blockchain governance compared to blockchain governance and classic business governance?

Enterprise blockchain systems, currently mostly private and permissioned, require an additional governance layer: business governance.

18 March 2023 478 1 View

Can I upload this document of 3 pages about Muskoxen (Ovibos moschatus) ?

Hi everyone, I want to know if it would be correct to add this document of 3 pages about Muskoxen (Ovibos moschatus) body weight references from the literature ? If so, what would be the best...

26 January 2022 4,842 3 View

Alternative to Hoyer's Medium?

Hello, I will mount to identify Embiidina, but the problem is that Hoyer's medium contains Chloral Hydrate, which is now forbidden in France. So I was wondering whether Dioni's mouting media...

28 December 2021 9,391 0 View

Is anyone aware of an interaction between PCL and chloroform?

We are spraying PCL using chloroform, and then dry in various conditions (vacuum or N2, 30°C to 50°C). At different time points, we measure the residual chloroform via headspace GC (150°C). When...

08 November 2021 5,825 1 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View