How to disolve GSK126 in DMSO And Captisol for an in vivo use in mice?

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Publishing a checklist?

Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...

01 August 2024 6,134 2 View

What are the most adequate analytical techniques for food contaminants analysis?

Good morning, Because food contaminants can be of different natures, is there a compendium of analytical techniques, standardized preferably, that can be consulted? Related to that question,...

15 February 2024 6,706 2 View

Potential for Y-H?

Does anyone know where to find the potential Y-H to perform MD simulations? I could not find it in the literature or in repositories. Many thanks in advance.

13 November 2023 7,268 0 View

Does genre imbalance impact Logistic Regression and Classification Models?

Seeking insights from the research community: Does the imbalance of textual genres within corpora, when used as an explanatory variable rather than the response variable, affect the performance of...

22 July 2023 1,790 3 View

How can technology (or innovation) negatively affect economic growth (or development), especially in countries with low technology levels?

Hello to all, I'm actually interested in the effects of technology on the level of economic development as measured by real GDP per capita. My results robustly indicate that in countries with low...

05 April 2023 4,498 11 View

What are the specific aspects of enterprise blockchain governance compared to blockchain governance and classic business governance?

Enterprise blockchain systems, currently mostly private and permissioned, require an additional governance layer: business governance.

18 March 2023 478 1 View

Can I upload this document of 3 pages about Muskoxen (Ovibos moschatus) ?

Hi everyone, I want to know if it would be correct to add this document of 3 pages about Muskoxen (Ovibos moschatus) body weight references from the literature ? If so, what would be the best...

26 January 2022 4,842 3 View

Alternative to Hoyer's Medium?

Hello, I will mount to identify Embiidina, but the problem is that Hoyer's medium contains Chloral Hydrate, which is now forbidden in France. So I was wondering whether Dioni's mouting media...

28 December 2021 9,391 0 View

Is anyone aware of an interaction between PCL and chloroform?

We are spraying PCL using chloroform, and then dry in various conditions (vacuum or N2, 30°C to 50°C). At different time points, we measure the residual chloroform via headspace GC (150°C). When...

08 November 2021 5,825 1 View

What would be the best optical technique to acquire images of a concrete slab in a high temperature furnace used for fire resistance testing?

During concrete fire testing, spalling of the concrete slab happens rapidly : pieces of concrete are expelled more or less violently. The visible optical images taken by endoscopic cameras are...

01 September 2021 7,409 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View