I use cell signaling Ab for the tissues from mice but couldn't get the band for p-NFkB/p65 and p-JNK MAPKwith dilution 1:1000 (primary) and 1:2500 (secondary).
I use cell signalling as well. I use 1:2000 for MAPK overnight incubation and 1:5000 for secondary. works good for me. But it also varies from cell line to cell line.