I found that liposomes from azolectin contain significant (up to 6-10 mM) amount of potassium which makes it a problem to study potassium transport
Hello! I would like to know if there is a way to flush/remove all the cells out of a tissue and keep only the extracellular matrix, in order to analyse its composition using e.g. proteomics....
14 February 2021 1,602 4 View
I want to find a database(s) or a method where I can find and bulk download all the rabbit MHC genes and domain annotation. In humans using Ensembl, all MHC genes have names and domains but in...
28 January 2021 7,722 2 View
Antibody based approaches aren't working as well as for other cell types, even IBA1 isn't working that well. Does anyone know of a histological/chromogenic stain for microglia? Or any other way?...
13 September 2020 5,927 3 View
Has anyone got any advice on how to reconstitute lyophilized material, contained in a 3mL glass vial, through an igloo cap, with only 125uL dilution buffer? I find that the volume is so small that...
21 August 2020 4,347 3 View
Does anyone have a simple and inexpensive protocol for supplementing seawater for seaweed in vitro culture? I am looking to replace the Provasoli medium with something simpler and cheaper (I have...
10 August 2020 302 3 View
Machine learning model, normally, is applied on new data for testing. If the accuracy of the model is below a certain threshold, the model is rejected. But why? This is just a random sample, the...
25 July 2020 8,615 4 View
Good afternoon, I was a performing a docking trough Autodock Vina, following a tutorial from the Scripps. I sent the corresponding commands to run the docking using a configuration file that I...
15 March 2020 2,328 2 View
I used to genotype my KO animals following this protocol: I extracted the DNA from the tail using a Sigma reagent and I performed the PCR reaction with a Promega Green MIX. It worked well, but due...
11 March 2020 7,926 1 View
I am using EEMS to analyze a sample of rodents (Calomys venustus) genotyped at microsatellite loci, living in an agricultural landscape. According to diagnostic scatterplots provided by EEMS, data...
04 March 2020 4,411 2 View
I want to conduct this research in the summer months, in order to estimate if high temperature and high air pollution are strongly correlated and if temperature is an estimator of the pollution....
09 February 2020 5,512 7 View
We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...
02 March 2021 6,036 1 View
I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...
01 March 2021 3,703 3 View
Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...
26 February 2021 5,525 3 View
I need to work with free lipids at increasing concentration (50%, 60% of the CMC ect.). I'm now working with POPC and POPG. To reach these concentrations I have to start from concentration over...
17 February 2021 1,676 3 View
Hi Everyone, I am trying to develop an SPE method for the vaccine which contains Matrix-M (saponin Quillaja Saponaria ) which is in nano-particle form and size is around 40nm. These nanoparticles...
16 February 2021 1,389 2 View
Activated carbon is prepared from biomass using KOH as the activating agent. What is the possible reaction between KOH and the biomass?
14 February 2021 8,095 4 View
pH 13 Buffer solution is prepared from a strong base sodium hydroxide and potassium chloride salt. However a buffer contain a weak base or weak acid with their salts. What is the purpose of the...
26 January 2021 9,154 1 View
I use the water method to extract the anthocyanidin from Peristrophe bivalvis (L.) Merr leave, then dilute 20 times with 0.025M potassium chloride, pH1.0 and 0.4M sodium acetate buffer, pH4.5...
20 January 2021 8,305 4 View
I want to find a GPC available solvent to dissolve both lipid (DPPC, 2 mM) and salt (NaCl, 100 mM).
14 January 2021 3,805 2 View
Hi ORO experts, I'm struggling with ORO background precipitates in arterial tissues. The protocol I use works very well on human atherosclerotic plaques which are full of lipids and I don't...
13 January 2021 7,110 1 View