Does anybody know the Qiagen's Puregen core kit recipe for cell lysis solution?

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How to fix cells with glutaraldehyde for immunofluorescent staining?

Hello, I am trying to fix primary cells with glutaraldehyde. I am trying to stain cell membrane proteins and I have not been successful with PFA and methanol fixation. Does anyone have a protocol...

17 June 2024 3,434 5 View

Immunofluorescence staining for only plasma membrane proteins?

Hello! I am having trouble staining for plasma membrane localization of a transporter. This transporter also resides intracellularly, however, I am only interested in staining its plasma membrane...

28 May 2024 8,249 5 View

Immunofluorescence staining and imaging of endothelial tubules?

Hello! I am trying to stain primary endothelial cells that have been grown on Matrigel to form tubules. I am interested in apical-basal polarity. The cells do form tubules but it is hard to image...

28 May 2024 5,855 4 View

The modal switching problem for multimodal robots ?

How does an amphibious robot with two sets of power units rationally switch between them? Or the modal switching problem for multimodal robots?

05 May 2024 4,625 2 View

What are the social functions of news discourse?

What are the social functions of news discourse? How it impacts social relationship, public opinion, social policy? What are the methods to research them?

18 January 2023 6,741 4 View

Some magnesium alloys will be darkened when immersed in corrosive solutions, why?

Some magnesium alloys do not corrode on the surface when immersed in a corrosive solution, but the surface is darkened, why?

20 July 2022 9,756 6 View

What causes long smear band in TBE-Urea gel?

This is the smear in 10% TBE-Urea gel. The lane in the picture was my control with only the oligo (57 nt). Last time I ran the same oligo with 15% TBE-Urea gel and got a clear band as it should...

27 June 2022 1,553 0 View

How is the review speed of Environment and Planning A?

How many days will cost on average for the review of papers submitted to this journal? If possible, does anyone know the approximate acceptance rate of this journal? Thanks a lot!

27 May 2022 9,387 1 View

Is PEGDA (DMPA) hydrogel antifungal?

I have read some literature saying PEGDA is a nontoxic biocompatible hydrogel. Therefore, I tried to grow Coprinopsis cinerea on a PEGDA (DMPA) hydrogel. I first made hydrogel with water solution...

04 November 2021 8,286 0 View

Are there any Earth mass Loading software written by Matlab?

Are there any Earth mass Loading software for culculating Love number, Green Functions and displacements written by Matlab? Most of the Earth mass Loading software analysis tools were written by...

27 October 2020 1,952 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View