Are there any Earth mass Loading software written by Matlab?

Are there any Earth mass Loading software for culculating Love number, Green Functions and displacements written by Matlab? Most of the Earth mass Loading software analysis tools were written by...

27 October 2020 1,847 1 View

How to determine one mutant which is overexpressioed or not?

We could get many mutants when we transform our target gene into the algae genome for overexpression or gene silencing .But how to check the mutant has overexpressed?By qPCR or Western Blotting?...

06 December 2018 5,166 2 View

Amidation in the graphene oxide can be easily get at room temperature about 273 k through stirring in the flask?

I want to make sure the correct condition about amidation in the graphene oxide , especially the critical temperature. through which I can contrast to other reaction

04 January 2018 604 2 View

How to use antibody to purify targeted protein for sequencing?

14 May 2017 9,521 3 View

Which parameter can represent the strain tolerance of thermal barrier coating (TBC)?

10 September 2016 4,067 2 View

If I add an electronic field on a 2D topological insulator bar in one direction, will the potencial difference appear in the other direction?

Suppose a topological insulator stripe, oppen boundary in Y direction, periodical boundary in X direction. If I add an electronic field in X direction, what will happen in Y direction?

24 August 2016 4,357 3 View

For one component it can have multiple peaks on the Raman spectrum. What could be the reason that only cause one of these peak intensity changes?

Raman spectroscopy. Raman peak Intensity changes. Inorganic materials.

11 May 2016 7,988 5 View

How can I seperate the near wall cell in Fluent UDF?

I want to modify the turbulent viscosity of standard k-e model in Fluent UDF, but the turbulent viscosity of wall function will change, too. And the change is not what i want.So i need to seperate...

16 October 2015 5,572 4 View

Could anyone suggest a protocol of extracting nuclear protein and detecting the protein's concentration?

Could anyone suggest a protocol of extracting nuclear protein and detecting the protein's concentration? I mean, I'd like to get respective nuclear and cytoplasmic protein from our -80 degree...

03 May 2015 889 7 View

How do I set a wall prandtl number in Star CCM+?

I know how to set turbulent prantl number in Star CCM+,but i have no idea to set the wall prandtl number. If use FLUENT, we can set like this:modle-viscous model-mocel constant-wall prandtl...

14 April 2015 1,719 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View