How to determine one mutant which is overexpressioed or not?

We could get many mutants when we transform our target gene into the algae genome for overexpression or gene silencing .But how to check the mutant has overexpressed?By qPCR or Western Blotting?...

06 December 2018 5,166 2 View

Amidation in the graphene oxide can be easily get at room temperature about 273 k through stirring in the flask?

I want to make sure the correct condition about amidation in the graphene oxide , especially the critical temperature. through which I can contrast to other reaction

04 January 2018 604 2 View

How to use antibody to purify targeted protein for sequencing?

14 May 2017 9,521 3 View

Which parameter can represent the strain tolerance of thermal barrier coating (TBC)?

10 September 2016 4,067 2 View

If I add an electronic field on a 2D topological insulator bar in one direction, will the potencial difference appear in the other direction?

Suppose a topological insulator stripe, oppen boundary in Y direction, periodical boundary in X direction. If I add an electronic field in X direction, what will happen in Y direction?

24 August 2016 4,357 3 View

For one component it can have multiple peaks on the Raman spectrum. What could be the reason that only cause one of these peak intensity changes?

Raman spectroscopy. Raman peak Intensity changes. Inorganic materials.

11 May 2016 7,988 5 View

How can I seperate the near wall cell in Fluent UDF?

I want to modify the turbulent viscosity of standard k-e model in Fluent UDF, but the turbulent viscosity of wall function will change, too. And the change is not what i want.So i need to seperate...

16 October 2015 5,572 4 View

Could anyone suggest a protocol of extracting nuclear protein and detecting the protein's concentration?

Could anyone suggest a protocol of extracting nuclear protein and detecting the protein's concentration? I mean, I'd like to get respective nuclear and cytoplasmic protein from our -80 degree...

03 May 2015 889 7 View

How do I set a wall prandtl number in Star CCM+?

I know how to set turbulent prantl number in Star CCM+,but i have no idea to set the wall prandtl number. If use FLUENT, we can set like this:modle-viscous model-mocel constant-wall prandtl...

14 April 2015 1,719 3 View

Why does the target protein level with siRNA-knockdown (on day 0) is reduced at day 2, rises back to control level at day 3 and drops again at day 4?

I performed a siRNA knockdown in vitro experiment. Target gene protein expression was decreased at 2 days after knockdown, however, it went back to normal level in compare with vehicle and ssRNA...

08 January 2015 9,787 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Where can I find atmospheric measurement profiles (temperature, density, etc.) between 300-800 km altitude?

I am investigating thermospheric mass density profiles between 300-800 km altitude. I would like to compare the variability with other parameters, including temperature, electron content,...

01 March 2021 5,902 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View