Zhiying Zou

4 Questions 2 Answers 0 Followers

Questions related from Zhiying Zou

Has any one compared Clontech's Lenti-X™ Concentrator and Ultracentrifugation?

I plan to do some Lentirivus Concentration. My virus will be used on cell line and HUVEC, so should not be very difficult. My goal is to get rid of the virus packing media and concentrate the...

03 February 2014 3,894 2 View

Does anybody know the Qiagen's Puregen core kit recipe for cell lysis solution?

I am running out of the cell lysis solution from the Puregen kit for DNA extraction from Qiagen. I am wondering if anyone knows their recipe?

16 December 2013 3,569 2 View

Has anyone used Neuroprobe, Inc's AP48 chemotaxis system to study migration?

I started using this system for breast cancer cell migration. I treated the membrane with 30ug/ml rat tail collagen overnight and used 8000 cell per well. After 5 hours migration, I cleaned the...

18 October 2013 2,531 1 View

Is CsCl purification of plasmids necessary for cell transfection?

Has anyone compared the effects of different plasmid purification methods on the mammalian cell transfection efficiency? I am trying to make MDA-MB-231 cell lines that stably express my proteins....

02 August 2013 247 15 View