Does anybody know of a technique to detect the protein carbonyl adducts?

04 April 2014 1 7K Report

Oxyblot from Millipore can detect carbonylated proteins/amino acids, but what I am looking to do is detect a carbonyl compound added to a protein so that the actual carbonyl group is no longer there and the carbonyl has been added to the protein.

Sanjay Premi

No, it is not AGE. It will be a chemically generated carbonyl of indoles and hydroxyindoles.

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