Detailed method for extraction of insecticide residue for HPLC.
First of all you should extract out bound residues of pesticides and its derivatives from the soil by solvent extraction method(Sohxlet extraction) and then check their solubility in solvents to make them inject-able into HPLC instrument. HPLC techniques are used with C-18 reverse phase column with UV detector for pesticide residue analysis.HPLC is the principal separation technique for identification of the pesticides in environmental samples and for quantitative analysis of analytes. At each stage of the HPLC procedure the chromatographer should possess basic skills that substantially help in accomplishing HPLC experiments correctly, to obtain reliable, repeatable, and reproducible results.Reversed-phase liquid chromatography (RPLC) with ultraviolet (UV) detection mode is most widely adopted, owing to its ability to perform efficient separation of very polar to apolar pesticides and the universal character of the detection mode. Therefore, a large part will deal with reversed-phase liquid chromatography/ultraviolet (RPLC/UV) applications showing its excellent separation performance and detectability of compounds not directly amenable to gas chromatography (GC).The more elective/sensitive detection modes, fluorescence detection (FD) and mass spectrometry (MS) as applied in pesticides residue analysis will be discussed with emphasis on the increasing importance of MS for identification and quantification of polar pesticides in various types of samples.
Dear Sir,
Kindly go through the link given bellow of ICAR-National Research Centre for Grapes.
http://nrcgrapes.nic.in (find publication box)
Definitely it is helpful to you.
Regards,
Dr. Mayur M. Aitawade
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