Does anybody know if CMV promotor in a DNA plasmid can be methilated? because there is not more expression of the target protein in mammalian cells?

More Elizabeth Reynaga's questions See All

About exosome isolation?

Hi, All, I am relatively new for exosome isolation. I used an izon column 70nm to isolate exosomes from differentiated neural cells, but the concentrations were pretty low after NTA analysis (I...

01 July 2024 2,558 0 View

Effective of teacher personality on children participation in activities ?

20 May 2024 7,434 0 View

Academic Term Papers and Seminar papers can i publish them?

I have lots of Academic Term Papers and Seminar papers which under go thorough literature reviews and are well reference but do not follow journal article guidelines. Can i publish them? Saba'atu

11 April 2024 1,729 2 View

What software can I use to compare two annotated genomes downloaded from NCBI database?

Which software to compare two genomes

09 April 2024 4,571 2 View

My son Rowan is studying 'Neuropsychology - Influence of Meditation on Adult Autistic Individuals' for his Research Project. Can anyone answer?

I have purchased a commercial grade EEG machine, for a reasonable price, in order to perform a study for my project. How familiar are you with less sophisticated EEG equipment? To what extent...

15 March 2024 9,449 5 View

US-based labs for hair cortisol analysis?

We are looking for labs based in the US that assess cortisol in human hair samples. We used to send samples to Mark Laudenslager's lab at the University of Colorado for analysis. We saw another...

29 February 2024 3,225 0 View

Using multiple role theory to examines leadership roles of school heads assigned in two schools?

i need to know what leadership roles used by the school heads who are assigned in two schools by using multiple role theory

12 February 2024 8,346 2 View

Please can someone show me in detail how I can calculate MAAT, MAGST and TTOP using live data from three different sites?

14 January 2024 1,617 0 View

Regarding expiration time for urine samples for drug screens is it always 2 hours or 4 if refrigerated ?

i am finding that drug court officers abiding by SAMSHA best practices protocol set up decades ago, have not been educated in interpreting results (they arent medical students) They look for the...

10 January 2024 440 2 View

Vector backbone alone eliciting a response in plant pathogen defense?

My colleague is trying to study the effects of a protein on pathogen inhibition in N. benthamiana. He is expressing it in either a pSITE vector or pCAMBIA1302 that had the GFP gene removed. They...

07 January 2024 3,413 1 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View