We had tested the expression of EGFP proteín in mammalian cells, before we got fluorescence, but currently the fluorescence resulted inexistent.
thanks a lot for you comments
Yes, CMV promoter can get methylated, especially in mammalian pluripotent ES cells and others. I'd recommend to use a plasmid that uses a EF1alfa based promoter.
Thanks Sara for your suggestion.
Thanks Sara!
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