Hi Adrian just a few quick questions:

Are the donor cells (the muscle derived stem cells) from the same sex as the recipient?

Also have the donor cells and the recipient cells been HLA typed? (I think you can see what I am trying to get at - that is in the absence of markers to differentiate cell types are there any markers to distinguish between the cells from two different humans).

This is of course assuming that the donor and the recipient are different people.

I am not too familiar with MDSC but I will look into it when I have the chance and get back to you.

Gary

Hi Adrian (me again) I came across a paper where they characterise the MDSC as being CD34+, CD56+ and CD13+.  http://mefanet.upol.cz/BP/2008/2/235.pdf

This is interesting as CD56 (and CD13)  is generally considered to be a lymphoid/blood marker

http://www.ebioscience.com/resources/human-cd-chart.htm

You might want to characterise the cells that you injected for some of these CD markers (you can buy conjugate ab's quite cheaply from BioLegends (no affiliation) to see if your cells express these (or others). On problem is that markers present on stem cells (such as CD34) can be lost after engraftment etc. so a negative result on an IHC is not 100% indicative of no cells being present.

Sorry I can't be more help,

Gary

Gary, MDSC were collected from the same patient...

Hello 

I agree with Gary Morley , a good answer

Good luck

Hi Adrian,

Of course if the transplanted "MDSCs" were mostly myoblasts (i.e. committed to a myogenic lineage) you can distinguish them from the resident cardiac cells because they are unable to transdifferentiate from skeletal to cardiac muscle (hence the disappointing long-term results from autologous human myoblasts transplantation after initial hope, in the early 2000). The transplanted cells will be expressing myogenin in single nucleated cells and/or the skeletal form of contractile proteins (myosin, TroponinT) in multi-nucleated myotubes.