How to calculate Reynolds number (Re) at curved surface exit?

Previously, I have used Reynolds number for dimensionless analysis for optimizing orifice shape (Computational study of fluid flow in tapered orifices for ne... ). I was hoping to do a similar...

21 January 2020 2,228 5 View

What are the basis of selecting a variety based on physiological WUE?

My question is which genotype/variety is best? The one with higher WUE (Pn/gs)? If so, then high WUE is associated with a lower yield. Is it means we are looking for the genotype which is best at...

09 December 2019 4,608 1 View

When should I add IPTG to a culture that I am planning to make electrocompetent?

I have inserted a plasmid (using a pET28a+ backbone, expressing the lac0 operator) into chemically competent BL-21 DE3 cells. I had selected for kanamycin and plated. I then picked a colony and...

16 September 2019 6,100 3 View

Is there a method for getting RNA into bacteria?

I know the methods available for transforming bacteria in order to take up foreign plasmid DNA. I, however, would like to utilize a non-viral method to infect my bacteria with foreign RNAs. I have...

19 August 2019 3,328 2 View

How to troubleshoot Gibson Assembly?

Hello, I have been working with Gibson Assembly in order to create three separate plasmids. I have ran PCR on pET28a+ and an already cloned plasmid containing two genes of interest. The GC...

05 August 2019 5,578 6 View

Can I use a dpn1 digest to remove template DNA after PCR with Q5 High Fidelity Polymerase?

I used a proof reading DNA polymerase, Q5, to amplify backbones and inserts that must have correct nucleotide sequences. I have already excised the bands at the appropriate lengths and have...

28 July 2019 3,989 3 View

Why are my cells not growing in LB but are growing on KAN plates?

The answer may be as obvious as there must be something wrong with the plates, but I am still perplexed. I have a plasmid system which has kanamycin resistance on the plasmid vector while...

14 July 2019 1,604 5 View

How can i distinguish between organic and inorganic material ?

i want to separate organic material and inorganic material.

10 January 2018 6,139 3 View

Which antibody is better DO-7 or DO-1 for p53 (tumor suppressor gene)?

I read some papers and most of them listed DO-7. Also, what other antibodies are better for p53 detection in terms of quality and reactivity and that are cost effective.

10 July 2017 9,185 6 View

Why many researchers use bead on plate weld type and not butt joint weld?

Various journal papers show that many researchers study the effect of process parameters by using bead on weld type and not butt joint weld.e.g. almost all papers on activated tig welding show...

18 April 2017 3,526 12 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

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03 March 2021 5,360 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View