I'm interested in any kind of preservation media for endometrial biopsy which could be stored at room temperature for at least up to 3 or 4 days.
I've tested RNAlater media, but it seems to fix the tissue, and although isolated cells are still stained with primary antibodies, i cannot recognize lymphocytes in the FSC-SSC plot because of the cell morphology transformation of the populations.
Dear Diana. Do you perform collagenase treatment on the sample before preserving? If it's single cell suspension it can easily be preserved in normal preservation medium DMSO (1part)+FBS (9 parts). RNA later is normally not recommended for flow cytometry.
Good luck
Thank you for your answer. Unfortunatelly our aim is to preserve the tissue sample without any kind of treatment until its arrival to our lab.
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