Dear All.,
I am interested in caveolin-3 mutations, and I was thinking about an in vitro approach by using organotypic skeletal muscle cultures. I could not find very much on PubMed, so I would greatly appreciate any suggestion.
Thank you in advance,
Best,
Veronica
Hi Veronica,
I tried few times in vitro mouse skeletal muscle culture, overall i test Soleo and EDL muscles in order to guarantee edium diffusion in whole tissue avoiding necrosis deeplu in the organotypic culture. I used in parallel standard plastic dishes or teflon grid to leave the muscle in supsesion not attached to the plastic in DMEM 10%FBS. I did not notice much differences, the muscles were alive, but the intercellular space was noteworthy increased do to medium soaking.
I hope this will help you,
good luck
Cesare
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