Anti-Src and anti-pY418 Src antibodies for IF?

Hi everyone, Does anyone have a suggestion for anti-Src and anti-pY418 Src antibodies that work well in immunofluorescence on cultured neurons? Thanks a lot!

26 January 2021 866 1 View

What is the impact of philanthropically-minded entrepreneurs in the institutionalization of social innovation?

Curious about the idea, that philanthropically-minded entrepreneurs can help to diffuse/institutionalize social innovation due to their belonging to at least two matured fields of business and...

12 August 2020 4,117 3 View

Are there any tools to extract data from facebook pages (posts, comments, etc.)?

Since it is not possible to work with Netvizz anymore I was hoping to find another tool which works similarly.

26 March 2020 959 3 View

Is there any reason why I can not order an oligo and use it directly as an insert for SLiCE cloning?

I was about to design the primers for SLiCE, when I realized the insert is so small I could order it as one oligo and it would not even be that expansive. As its quite some time ago, that I used...

18 March 2020 2,996 3 View

Sars-cov-2 (Covid-19): qPCR or serological antibody testing - is there information?

So far I only read about PCR testings to confirm a patients infection. Is somebody aware of other diagnostical testing options? E.g. for EBV (Ebstein Barr Virus) exists a serology testing method...

12 March 2020 3,965 6 View

Which problems in fire fighting could be addressed with mobile robots? For which industrial scenarios would such a fire fighting robot be helpful?

I am looking for narrowing down the topic for my master theses. Real industrial problems to be solved with innovative technologies are the most interesting topics.

30 April 2019 5,506 6 View

What are some good examples (case studies) for using open badges in higher education?

I am writing a post about open badges, and researching some examples.

02 April 2019 4,484 2 View

Can anyone suggest a software for designing primers and internal oligos on bisulfite converted DNA, instead of Beacon Designer?

07 June 2018 6,063 2 View

Programs to open .gel files to quantify Western Blot images other than ImageQuant TL?

I don't have access to ImageQuant TL as you need to pay for a license, are there any similar programs that are free for opening .gel files?

19 April 2018 6,565 2 View

Suggestions for reliable online questionnaires to evaluate neuropsychiatric disorder symptoms?

Can anyone recommend validated online (web-based) questionnaires to evaluate neuropsychiatric disorders/symptoms?

09 October 2017 4,684 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

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02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View