Is separation of porphyrins on a hypercarb (PGC) column possible?

Hello, I am struggling with separation of porphyrins (i.e. 5,10,15,20-Tetrakis(pentafluorophenyl)porphyrin, protochlorophyllide, chlorophyll a and b) using a hypercarb (porous graphite carbon)...

31 December 2018 318 4 View

What is the practical difference between ODS-A and ODS-B in Teknochroma Tracer Excel HPLC columns?

Does someone have any practical experience with those columns?The producer suggest ODS-B are more suitable for hydrophilic analytes, but without any concrete information.Can they be used...

04 May 2017 7,793 4 View

Protocol for norflurazon detection in plants?

I am unable to find a protocol for norflurazon detection in plant tissue. I have only encountered works concerning quantification of norflurazon in soil samples (Essington et al., Comun. soil....

10 November 2016 2,797 5 View

Simple spectrophotometric method for carotenoid content determination?

06 July 2016 2,933 3 View

Will dextranase EC 3.2.1.11 hydrolyse DEAE-dextran or dextran sulfate?

We are working on dextran quantification in urine samples using HPLC-MS. Our first step is dextran hydrolysis using a dextranase (EC 3.2.1.11) from Penicillium sp. We need a method to control...

07 August 2013 5,215 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View