Hi all. Is anybody of you experienced with measuring folate levels in mouse plasma? If yes, which method do you usually use and what levels do you expect in a healthy adult mouse? Thanks lots for your help!
Dear Dr. UIrikeWeber
Look to referances below may helpe you .
. Horne DW, Patterson D. Lactobacillus casei microbiological assay of folic acid derivatives in 96-well microtiter plates. Clin Chem. 1988;34:2357–9. [PubMed]
Spiegelstein O, Merriweather MY, Wicker NJ, Finnell RH. Valproate-induced neural tube defects in folate-binding protein-2 (Folbp2) knockout mice. Birth Defects Res Part A Clin Mol Teratol. 2003;67:974–8. [PubMed]
Good luck
I wish the below link could help,
1)http://www.nejm.org/doi/full/10.1056/nejm199905133401901#t=article
2) http://cebp.aacrjournals.org/content/8/9/825.short
3) .http://circ.ahajournals.org/content/94/10/2410.short
All the best
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