If the intensity of a peak is lower and/or the linewidth is higher, does it mean the specificity/affinity of the ligand is higher for that residue?
Dear Paul,
essentially all explained by the McConnel equation https://groups.chem.ubc.ca/straus/Nlecture3.pdf
Alfred
Alfred Ross thanks for the reply.
So, if I understood correctly, the answer is probably yes.
The term peak intensity has no meaning in NMR signals. Peak height and, or peak amplitude are less ambiguous. The peak height is a function of signal amplitude and peak width at half height. Peak amplitude is traditionally estimated using frequency domain integration. The integration estimate is commonly called peak area. A broad signal can have the same amplitude as a narrow signal. Peak area only estimate signal amplitude for well-separated signals and when a constant in the data (baseline offset) does not affect the frequency-domain spectrum base line.
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