What is channel gain Formula for Free Space, Rayleigh and Rician cases, for Matalab Channel modelling?

Hello, I would like to model a channel but I have to consider different cases. I need to start from matematical basis of formula, to work with matlab, can anyone could help me?

26 December 2020 9,602 3 View

How can I model a simple communication channel considering Reconfigurable Intelligent Surfaces using MATLAB?

Hello, I would like to model a communication channel, but considering in my scenarios Reconfigurable Intelligent surfaces. Does anyone have any ideas?

18 December 2020 8,137 2 View

Should these surfaces be hydrophobic?

I am trying to coat PDMS and Polyimide with (3-mercaptopropyl)trimethoxysilane and (3-glycidyloxypropyl)trimethoxysilane respectively. I was wondering whether these surfaces should exhibit a...

29 October 2020 5,970 4 View

This question has been answered, delete?

I have found the answer

11 September 2018 3,842 1 View

Ligation between oligo and double strand DNA

Hello, I have a solution wih a DNA fragment with a sticky-end cut. It is possible ligate the oligo to this extreme without anneal it with the complementary? Thnak you

15 May 2017 6,321 6 View

Rocking curve scan XRD ?

24 July 2016 2,892 1 View

Can oscillations in temperature change the measured cell capacitance?

I'm currently patching pancreatic beta-cells using a HEKA amplifier to measure change in cell capacitance due to exocytosis events. I keep on having big oscillations appening every few seconds,...

06 June 2016 5,933 4 View

Amygdaline: effective or not against cancer?

several old studies says no, more recent studies says yes. Robert Bell in 1870 used raw salad to increase amygdalin in cancer patients and used saliva to detect the presence. Several sources say...

02 June 2016 8,606 4 View

Ligation of multiples restriction fragments

Hello, I have a mix of 10 fragments generated with 2 endonucleases (1 produces blunt end and other , PSTI, produce overhang ends): each fragment has a blunt and and overhang end. I want ligate a...

17 February 2016 3,652 1 View

Does anyone know a specific inhibitor of Exportin-5?

Does anyone know a specific inhibitor of Exportin-5? miRNA transport

12 January 2016 1,754 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View