Does anybody have a protocol for reverse transcription?

01 January 2014 1 5K Report

I have tried various different protocols for reverse transcription with random primers and I don't see any obvious difference in the final qPCR reaction. Any suggestions?

I would also like to know if DTT is needed if we use Rnasin and not rnasOUT.

Jeremy Dufourt

So i finally have answer from promega dtt is not needed for rnasin working

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