Jeremy Dufourt

8 Questions 11 Answers 0 Followers

Questions related from Jeremy Dufourt

Immunostaining in SSC 2X buffer?

Hi, I would like to know if some people use SSC 2X buffer for immunostaining, i should do an experiment where i cant use PBS in drosophila embryos. If you have advice or knowledge about Staining...

04 April 2019 2,740 9 View

Drosophila embryo permeabilization to deliver drugs and image at the same time?

Hi all, I want to image drosophila embryo with drug treatment, of course the major problem is the viteline membrane, and the survival of embryos to permeabilization. I wonder to know if someone...

03 March 2019 304 2 View

Immuno smfish drosophila?

Hi, I am coming here because i tryied several protocol To do an immuno single molecule fish in drosophila embryos and it works with some probe by doing fish follow by immunostaining but i recently...

01 January 2019 5,331 4 View

Visible immunostaining using DAB ?

I wonder to know if immunostaining using DAB is sensitive to amount of tissues as il use drosophila embryo i cannot know exact amount of tissues and for some mutant i have very few embryo. Is this...

02 February 2016 242 3 View

How to normalize an RNA immunoprecipitation?

I have a problem with the logic of an RNA IP normalization. I am comparing two RNA binding proteins tagged with GFP, one is an isoform of the other where I mutated one binding site. My IP is done...

05 May 2014 2,021 3 View

RNA extraction question

I wanted to know if it is a problem to precipitate RNA in isopropanol at 13000g because in a lot of protocol says to precipitate at 12000g.

01 January 2014 3,274 4 View

Does anybody have a protocol for reverse transcription?

I have tried various different protocols for reverse transcription with random primers and I don't see any obvious difference in the final qPCR reaction. Any suggestions? I would also like to...

01 January 2014 5,132 1 View

When you extract RNA with Trizol you should break the RNA if you vortex too much ( >1min.)?

If it is the case is it a problem for qrtPCR after that as I do reverse transcription with a random hexamer. Thanks for your answer.

01 January 2014 4,865 4 View