Drosophila embryo permeabilization to deliver drugs and image at the same time?

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Immunostaining in SSC 2X buffer?

Hi, I would like to know if some people use SSC 2X buffer for immunostaining, i should do an experiment where i cant use PBS in drosophila embryos. If you have advice or knowledge about Staining...

03 April 2019 2,683 9 View

Immuno smfish drosophila?

Hi, I am coming here because i tryied several protocol To do an immuno single molecule fish in drosophila embryos and it works with some probe by doing fish follow by immunostaining but i recently...

31 December 2018 5,257 4 View

Visible immunostaining using DAB ?

I wonder to know if immunostaining using DAB is sensitive to amount of tissues as il use drosophila embryo i cannot know exact amount of tissues and for some mutant i have very few embryo. Is this...

01 February 2016 188 3 View

How to normalize an RNA immunoprecipitation?

I have a problem with the logic of an RNA IP normalization. I am comparing two RNA binding proteins tagged with GFP, one is an isoform of the other where I mutated one binding site. My IP is done...

04 May 2014 1,942 3 View

When you extract RNA with Trizol you should break the RNA if you vortex too much ( >1min.)?

If it is the case is it a problem for qrtPCR after that as I do reverse transcription with a random hexamer. Thanks for your answer.

31 December 2013 4,814 4 View

Does anybody have a protocol for reverse transcription?

I have tried various different protocols for reverse transcription with random primers and I don't see any obvious difference in the final qPCR reaction. Any suggestions? I would also like to...

31 December 2013 5,078 1 View

RNA extraction question

I wanted to know if it is a problem to precipitate RNA in isopropanol at 13000g because in a lot of protocol says to precipitate at 12000g.

31 December 2013 3,228 4 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS?

I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...

01 March 2021 736 3 View

How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View