Does an upregulation in PPAR mRNA mean that PPAR is in its active form?

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How to identify if a material is behaving like an anode or a cathode?

when we perform a cyclic voltammetry study, how can we conclude if a particular material is behaving like an anode or cathode with the help of that CV plot?

20 June 2024 1,453 3 View

Which protocol to follow for TBARS assay?

I want to measure MDA concentrations using the TBARS assay in in vitro cell lines. Currently, I am following the protocol as described by (Aguilar et al., 2022) in their paper "Evaluation of...

12 May 2024 7,541 0 View

Is it possible to get redox peaks in the negative potential window of CV plots?

The active material is a MXene/sodium based composite. All the active materials used as electrode material for SIBs shows redox peaks in the positive potential window but i have got in negative...

17 April 2024 7,024 0 View

For a multi-step treatment on in vitro cell lines, do I replace the 1st treatment with the 2nd treatment or do I add the 2nd treatment to the 1st one?

I am currently setting up a two step treatment on HepG2 cells in a 96 well plate, using an antioxidant and a drug. Majority of the literature that I have consulted mention a pretreatment with the...

26 February 2024 5,970 1 View

How much R square value should be there to validate preparative chromatography DoE model?

I am working on mAb purification DoE, wherein i am getting about 0.8 R square value.

16 October 2023 1,384 2 View

How much rotation is acceptable in pediatric supracondylar fracture?

Pediatric supra condylar fractures sometimes show rotation after fixation. It may be significant in some cases. What is the normal range. What is significance of gordons classification.

11 October 2023 3,899 1 View

Where is the list of UGC approved pharmacy journals available?

08 October 2023 8,018 3 View

How can I download and install quantum espresso for windows ? I want to learn QE, any one has guiding video link?

Guide

03 September 2023 139 5 View

How would you separate hydroquinone, cyclohexanone and cyclohexanol by GC ?

22 August 2023 1,692 1 View

How to test reliability and validity of a questionnaire that has yes/no type of questions and few open ended questions?

My questionnaire aims to find if the participants have been a victim of click fraud in online advertising by asking questions related to various metrics that are used to detect the occurrence of...

23 July 2023 6,096 0 View

Is there an English Translation of the Carl Moller text: ZUR VERGLEICHENDEN ANATOMIE DER SILURIDEN?

I recently came across an anatomy text by Carl Moller that was published in 1915 but it is in German or Dutch neither of which I can understand. I would like to know if there is an English...

10 August 2024 4,347 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

How to conduct a sensitivity power analysis for Kendall's Tau?

Is there a straightforward way to conduct a sensitivity power analysis for a Kendall's Tau correlation? I was considering using the sensitivity setting and "Correlation: point biserial model" test...

28 July 2024 6,133 8 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View