What do you think of using any nanoparticles in the treatment of coronaviruses, especially if produced less than the diameter of the virus?

a novel path to eliminate coronavirus

23 March 2020 5,943 40 View

How to calculate current density of plate electrod?

I have 2 Aluminum plate electrodes anode and cathode. each plate of 2.5x3x0.1 cm dimension, and connected to a power supply. I want to use this electrodes in electrochemical processes. Please, how...

02 December 2019 4,760 3 View

Hello, please i need explanation about this picture from kidney of medaka fish stained with Mallory stain for detection of colagenous fibres?

Why inside the tubular segment cells take the green color and appear like the shape of the nucleus insude their cells...is it may be vacuoles take green with this stain?..please i need help

22 February 2019 4,206 3 View

How can calculate a dose to rat copared to human?

Please, i need a help. If i have omega3,6,9+ingrediants in soft gel capsule (each capsule contain 3600mg) and thr recommended dose for human from 3000 to 5000mg daily.how much dose can i give it...

03 November 2018 840 3 View

How to import cv2 into anaconda (spyder), Python 3.6 ?

I'm using python 3.6 on spyder 3.3 (Anaconda) and I use lib cv2 but it doesn't work. It gives me " DLL load failed: The specified module could not be found " When I did search about this problem I...

09 August 2018 2,510 22 View

How can I get from a Thermal face image the nearest facial biometrics to Polarimetric-Thermal face image ?

As all of my knowledge it's difficult to get Polarimetric-Thermal Face Images Database (If anyone knows a public one mention please). So, how can I use (Enhancement or Alg) to get the nearest...

26 July 2018 5,372 1 View

How to get the highest yield when extracting a plasmid of 45 Kb?

We have a bacterial strain with multiple plasmids, I m interested in one plasmid which around 45kb, no selection marker is available for this plasmid thus not conjugated nor transformed so the...

06 March 2018 5,280 4 View

What are the most important reasons for using HACCP ?

What are the most important reasons for using HACCP ?

11 April 2017 6,393 14 View

Is anyone interested in Reuse Seafood by-product as an ingredients of functional food?

04 December 2016 335 8 View

How to compare result from model with others from different model and for different dates?

I have a set of results from mathimatical model along 1000 m for every100 m and for different dates. What is the best way to statistically compare the performance of this model with the standard...

01 May 2016 1,636 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View