I want to know if there is any evidence of changes in mitochondrial gene expression or protein levels in adipose tissue or liver due to a i.p. dose of insulin 20 min before the sacrifice of the rat. When I say mitochondrial gene expression, I'm referring to genes involved in biogenesis or mitochondrial dynamics like pgc1, tfam, nrf1, mfn1,2 fis1, or drp1.
Probably not, but I do not remember if anyone did something like this experimentally.
Thanks a lot Laszio G Mesazaros. I will keep digging into it.
Gene expression changes are unlikely to be detected at significant levels on this time-scale. Hours would be required. Post-translational modifications are the usual detectable changes that occur within minutes of an insulin treatment, eg. Phosphorylation of signalling intermediates.
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