Hi all,

I guess everything is in the title: would you expect (or better yet was it shown) that 3´RNAseq, where only the last 50-75 BP of the transcript are sequenced, to be affected by RNA degradation, the same way traditional RNAseq is? And thus, would you define the same RIN cutoff for a 3´RNAseq and a traditional RNAseq experiment?

Looking forward to your answers!