Do you know whether a software for designing degenerate primers for LAMP PCR even exist?

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Causa of WRF-Fire crash: NaN detected in line 152?

Hello everyone, I am trying to run the Fire module of WRF 4.2.2 using real data (GFS 0.25), and I get the the following error message after running wrf.exe: Timing for processing lateral...

15 February 2024 4,695 0 View

RNAseq from fixed T cells?

Hello everyone, I would like to know if anyone has experience in performing RNAseq from lymphocyte suspension (Sorter, MACs, etc.) from fixed and permeabilized samples? I was thinking of sorting...

05 February 2024 2,914 0 View

What are good protein binding site prediction softwares?

Hi, we've currently been researching about mutant HIV-1 RT, and we're having a hard time figuring out what software to use in order to determine the protein's binding site (so we wouldn't have to...

24 September 2023 5,723 0 View

What's the best way to quantify Drosophila egg laying? How many eggs should I expect in a first cross?

Is there any software or tool? Is there any variation in the reproduction rate of different species of Drosophila? Thanks for your help.

09 December 2022 6,127 0 View

Which concentration of HCl should I use to observe the carboxyl band in a FTIR spectra?

Hi, I'm functionalizing crystal nanocellulose (CNC) in order to increase the number of carboxyl molecules on the surface, however, the carboxyl signal on the FTIR is barely visible. According to...

11 September 2022 7,764 0 View

Which concentration of HCl should I use to observe the carboxyl band?

Hi, I'm functionalizing crystal nanocellulose (CNC) in order to increase the number of carboxyl molecules on the surface, however, the carboxyl signal on the FTIR is barely visible. According to...

11 September 2022 3,999 2 View

Can someone help to identified this specie of polychaeta ?

I only know that belong to Nereidae family

19 June 2022 9,083 3 View

Could I use a BAM file output to make a alignment with others FASTA references?

I am wondering about to use a BAM file output as a input to make a alignment to others FASTA reference genome. I have been reading how to get a FASTA formand from my BAM file but I am not sure if...

23 February 2020 9,878 5 View

How to dissolve 44.46 g of Aluminium sulfate in 100 ml of double distilled water at room temperature?

I need to prepare a 1.3 M Aluminium sulfate (anhydrous 99.99% - metal basis) solution, so based on its molar mass (342 g/mol) I need around,44.46 g of Aluminium sulfate in 100 ml of water to make...

27 November 2019 8,657 1 View

How can I set a gradient anisitropy with OOMMF?

In oommf manual just i can set uniaxial anisotropy or cubic anisotropy, but how can i set a gradient anisotropy for example https://ieeexplore.ieee.org/abstract/document/7932946 (Micromagnetic...

01 September 2019 8,602 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Identify the eight key processes of excellent supply chain management and discuss how each of these processes impacts the end customer?

Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26

04 August 2024 9,919 4 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View