How to quantify relative amounts of a substance with immunostainings through confocal microscopy when samples have high autofluorescence?

I am working with plant tissues, buds specifically, fixed in glutaraldehyde and embedded in LR White resin. I am working with the primary antibodies LM19 and LM20 targeting pectins. I am using a...

21 September 2020 5,213 6 View

What tools do you use for development and training on a remote machine?

Hi, I currently use vscode with the remote add-on for this and a ssh session with tmux. With this setup I can edit files, debug and run scripts remotly. What I use: Python, C++, ROS, Gazebo,...

14 May 2020 3,760 3 View

With SOLID185 microplane damage model. How do I solve my error in Ansys??

In the last weeks I have worked the damage model of the element SOLID65, but it has not allowed any results, so I decided to work with the element SOLID185, with the recommendations of the...

07 March 2019 5,662 5 View

How to create a variable with specific quantitative shocks in STATA?

I am currently working with a time series database, and I would like to study the effects of a sudden increase in the price of a good. I have some dates and some specific shocks ( an increase in...

14 February 2019 6,209 2 View

Asking for kit of DNA extract of tissue fish?

Hello reaserchers i am trying to extract DNA of fish tissue in the tail on the lungfish so i would be happy if someone did a extract of tissue please email me [email protected]

20 November 2018 7,506 1 View

Someone know how to prepare cocoamidopropylbetaine with solid content over 40 %?

i have been trying to prepar cocoamidopropyl betaine with solid content above 40 % but i only have reached 37 % of solids. Every time i try to increse the solids content it forms a gel.

10 April 2018 2,318 1 View

Can you use a pair of primers, used for Taqman qPCR, in SYBR qPCR without probe?

I am trying to use a qPCR for diagnostic of a phytopathogen. I have a pair of primers, designed and used with a probe in a Taqman qPCR for detection and diagnostic. I got the pair of primers but...

10 January 2017 8,351 3 View

Nature of time in QM ?

Inherent probabilistic nature of measurement in QM seems to impose a distinction between past and future and so a definite direction of time, similar to that occurred in statistical mechanics....

08 April 2016 2,300 3 View

Which is the true Nature of time in Quantum Mechanics ?

Inherent probabilistic nature of measurement in QM seems to impose a distinction between past and future and so a definite direction of time, similar to that occurred in statistical mechanics....

08 April 2016 597 4 View

Can someone tell me how to reduce the precipitate (particles) of hematoxylin and eosin staining in histological sections?

There is much precipitate (particles) of hematoxylin and eosin in histological sections and we can not distinguish well the structure of epithelial cells. Staining was carried out automatically on...

24 February 2016 4,339 11 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View