Greatings, I am looking for a manual or book that includes the metodology to determine microbial biomas and fungal to bacterial ratio using a microscope.
Thank you in advance.
Is it measured from the apical parts to the center of the microalgae ? or do you have another ways to measured it? I attach an image to explain thanks
12 February 2024 6,924 3 View
I've used the "dist.point" function in the mFD package to get the data.frame of pair to pair comparison plots, but I'm wondering if it's possible to get the total, nestedness, and turnover beta...
26 November 2023 2,719 0 View
I need help with a PCR. I have the following primers mecA-F 5′AAAATCGATGGTAAAGGTTGG-3′ / mecA-R 5′-AGTTCTGGCACTACCGGATTTTGC-3′. I made an initial PCR with 12.5 of GoTaq Green Master Mix, 2x, 0.8...
05 October 2023 7,228 2 View
I am in the process of conducting an electrophoresis and I have a Safer dye at my disposal. However, I am uncertain whether it would be more suitable to combine the Safer dye with the 6X DNA...
30 August 2023 596 3 View
Is it possible to employ the boiling extraction protocol for fungi? I am working with the ATCC strain of Candida auris and conducting environmental research on this fungus using swabs. I would...
17 August 2023 9,381 2 View
I prepared TAE for running electrophoresis and would like more information about how long of an expiration period I can consider.
15 August 2023 4,785 3 View
Usually, I use a time of 120 minutes and 120 V, but I am testing and standardizing new primers and I realized that perhaps the sample might have run off the gel. I would like to know if you use...
15 August 2023 7,093 1 View
I would like to know how I can find out the size of the amplicon from my primers. I have these primers: Forward: GCTCCGCAGCCTGCATGGA Reverse: ACGATGCCGCCATCCTCCT. I saw the suggestion of putting...
12 August 2023 238 12 View
Hi, my intern and I were working on imaging V. corymbosum buds in a confocal microscope (LSM900). Our plan was to have the same settings for all the images, but we changed (by mistake) the gain...
30 July 2023 9,030 2 View
Hi everyone, i have a Z score of -11,22 in my protein model. This is good or bad? Thanks for your answers
29 July 2023 970 3 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?
07 August 2024 2,132 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Hello everyone I am looking for one or some books for propertes and behaviors of stochastic fibre orientation composites. unfotunately I could not find any suitable reference for thias by...
04 August 2024 3,461 3 View
We are planning to establish a microbial laboratory in our research center and are actively seeking the best disinfection methods. If anyone has any recommendations, please let us know. Thank you.
04 August 2024 1,973 1 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...
03 August 2024 6,028 4 View
Suppose I am studying the construct of "Academic Motivation" (latent variable) in students, which is measured through three observed variables: "Intrinsic Motivation," "Extrinsic Motivation," and...
31 July 2024 3,913 6 View