Hi
I am working on MDA-MB-468 EGFR(+) and i want to find the same cell line but without EGFR (-) ?
I did some research on NCBI but i didn't find an article when someone use it.
If anyone have this information thanks a lot !
As far as I am aware there is no such line commercially available. (That isn't to say it doesn't exist though!) You can buy EGFR+ and EGFR- HAP1 cells if that can also help to answer your research question.
Alternatively, with the advent of CRISPR it isn't very difficult to genetically modify your cell line of choice. You could always design some gRNA against EGFR and make the cell line yourself.
As Dr. Elliot Macleod suggested, CRISPR-Cas9-based knockdown of EGFR is suitable. Other than genome editing, you can also suppress the EGFR expression by small-interfering RNA (si-RNA) or short-hairpin RNA (sh-RNA).
Furthermore, you should use more than three cell lines including
MDA-MB-468 depending on your research hypothesis.
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