What are the "units" in RNA seq data processed by RSEM?

Hello! I've been using gene expression data from the TCGA, where the RNAseq is processed using RSEM. I did a few graphs such as boxplots showing the expression of different genes in normal vs...

14 October 2020 2,721 2 View

Which is best way to purify my His-tagged protein after eluting from a Ni NTA column in denaturing conditions (urea 8M)?

I have cloned two versions of my protein of interest to produce antibodies in mice. It is not soluble in native conditions, so I am trying to purify it in denaturing conditions with urea 8M. Now,...

18 February 2020 7,305 3 View

Multi-layer wells "robbed" of pumping rates?

By the way, I send attached the workflow I've performed. It is strange that in the Link to parameters window, in the Topology processing, instead of "no topology processing", now what I get is "no...

17 February 2020 8,662 3 View

How to reject authorship of some papers?

You added in my published paper list some article that don't belong to me. The author has the same family name as me, but is being active in a total different area of research. Thank you in advance

24 January 2019 5,795 3 View

Is there an easy way to count the diatoms in a rock sample in a short amount of time?

Hello! I have a bit of a struggle with my diatomites samples. I have tried all the methods to break the diatoms from the rock but I got a negative results (they are highly silicified) and I want...

04 September 2018 6,353 3 View

Any recommended (free) software to rectify and post-process coastal oblique images?

We have a fixed time-lapse camera (RGB) located on a hill oriented towards a sandy coastal area. Images are taken every 30 min. By using a set of ground control points (GPS) we aim to rectify and...

24 October 2017 4,632 5 View

Does Pfu Taq polymerase generate 5'P end?

Does the PCR product amplified using pfu polymerase have 5' phosphate groups to use for blunt cloning?

22 June 2016 1,058 3 View

How can I break the exopolysaccharide capsule of the mucous colonies to extract the RNA and DNA?

Hello to everyone! I'm working with strains of Acinetobacter baumannii resuspended in NaCl 0.45% (8 McF in 2 mL). Colonies of Acinetobacter baumannii are mucous so that they can not be separated...

15 June 2016 6,500 1 View

Why do I have a smear in gel for my bacterial RNA sample?

I have a question.....i have made a agarose gel of bacterial RNA and i have obtained a smear....what's happened? the RNA is 588mer. Moreover, i have done a Real Time PCR of these RNA without...

25 October 2015 7,189 4 View

Large-scale reaction with TnT T7 quick coupled transcription translation system (Promega)?

I wonder if the TnT T7 quick coupled transcription translation system (Promega) works in a large-scale reaction. They suggest a final volume of 50 ul and I would need a final volume reaction of...

17 August 2015 8,270 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View