Do you know a different way to assay Halliwell–Asada cycle than spectrophotometric methods?

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How to eliminate the carry-over effect in GC-MS/MS system?

Hi, I am a PhD student working at Jagiellonian University, Faculty of Chemistry. My work is related to the development of new analytical methods for the quantification of short-chain fatty acids...

22 July 2024 5,492 6 View

Volcanic Ash in modern concrete and cementitious materials?

I am writing a paper summarizing my research results on the possibility of usage of volcanic ash in modern cementites materials on the example of the tests of composits with particular (Japanese)...

21 February 2024 5,797 0 View

How to calculate DBH for trees with multiple dead and live stems in the same tree?

I am trying to calculate DBH (diameter at breast height) in order to determine the demography and basal area of trees. However, some of the trees have multiple stems which makes it difficult to...

23 January 2024 845 0 View

ReserchGate misatributes some citations to me. What should I do?

The site shows that my works are cited in some papers, however when I checked those papers, I found no sign of being referenced (on the other hand my work is referenced in completely different...

22 January 2024 3,766 2 View

Are you interested to collaborate within the new research project "Education in the AI era"?

I'm looking for hard-working researchers for the new research project "EDUCATION IN THE AI ERA." I would like to create the survey on Google and do international research. If you are interested in...

06 January 2024 5,488 21 View

Why I'm losing plasmid after digest with restriction enzymes?

Hi everyone, I'm currently working with a plasmid called pBI101, I isolated this plasmid from a plate then I did the extraction with mini prep protocol and cut it with SmaI at 25°C incubation...

17 November 2023 6,980 3 View

How to desing “splinkerette” adapters for TraDIS (transposone) libraries?

I'm trying to optimize TraDIS for our model bacterium and I am having some technical problems with the DNA preparation for the sequencing step. I'm struggling with efficient PCR enrichment of...

12 September 2023 5,836 0 View

Transfection of adherent primary cells using Neon transfection system - the best electroporation settings to start with?

Hello everyone, I am trying to make a CRISPR-Cas9 knockout on lung cancer primary cells using the RNP complex. I am using the Neon Transfection System but so far it failed - all the cells that...

30 August 2023 8,660 2 View

BDNF unit conversion - does anyone know the formula?

Hi, does anyone know the formula for converting the BDNF value in biological fluids (pg/ml to ng/ml and vice versa)?

24 July 2023 1,280 1 View

How do I get factor loadings for single indicator latent variable in SEM?

Hi, I'm doing CFA using SPSS Amos and I have two variables with single items each. After reviewing previous discussions, I've fix the loadings and error variance to 1,1. If I put error variance to...

24 June 2023 3,631 3 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View