Joanna Chojak-Koźniewska

8 Questions 8 Answers 0 Followers

Questions related from Joanna Chojak-Koźniewska

H2O2 assay in plant suspension cultures?

Does anyone know if there is any non-fluorescent but acurate methos for h2o2 assay in plants suspension culture? Thank you for help.

07 October 2022 912 1 View

Does anybody design a PCR reaction to quantify the number of mutated gene copies, i.e. design a PCR specific only to the mutant DNA fragment?

I want to determine the level of crossing of one rapeseed variety ("A") with another variety ("B") that has a known SNP in the target gene. If it comes to a crossing, then in DNA isolated from...

28 June 2017 6,712 9 View

Can anybody tell me if RNAiso Plus (Total RNA extraction reagent, TaKaRa) is OK for RNA isolation?

Does anybody use this kit? The main advantage is its low price, but is it comparable to e.g. thermo?

18 January 2016 1,849 4 View

What is the difference between the Duncan test and Tukey test and which of them is more suitable in my case?

Which test is more suitable for plant science? I have plants which are treated with two different chemicals, so I have control plants, and two variants with above mentioned chemicals. Those plants...

27 February 2015 6,280 5 View

Does anybody know a negative effect of salicylic acid on plants?

e.g. high concentrations of SA or interaction with abiotic stress factors and promoting its negative effect? Many authors indicate that SA has a beneficial role in plant-pathogen interactions or...

13 January 2015 7,130 7 View

Does the enzyme Ascorbate oxidase only work in apoplast in plants?

Do you know any relationship between the decrease in dehydroascorbate concentration and increase in ascorbate oxidase activity?

13 March 2014 1,537 0 View

Does anybody know how to successfully assay the activity of malate dehydrogenase in cucumber plants?

I've tried many times, but unsuccessfully. I performed homogenisation in 50mM Tris buffer pH 7,6, which contained 1mM EDTA, 1mM MgCl2, 10mM β-merkaptoetanol, 1mM DTT, 0,5% PVP. Also, I used PMSF...

08 January 2014 4,213 0 View

Do you know a different way to assay Halliwell–Asada cycle than spectrophotometric methods?

For example, the activities of ascorbate-glutathione cycle enzymes, namely ascorbate peroxidase (APX, EC 1.11.1.11), monodehydroascorbate reductase (MDHAR, EC1.6.5.4), dehydroascorbate reductase...

09 June 2013 1,895 2 View