I want to isolate mesenchymal stem cells from human bone marrow
Do you have troubleshooting and GMP condition for mesenchymal stem cell isolation from human bone marrow?
It is pretty straight forward procedure if you know cell culture technique. I am enclosing a paper for you to read, if you have any question, please email me.
I have a Karl-Fischer reagent (for volumetric titration, which is a one-component reagent) with a titer of 5mg/ml. All references unanimously say the Karl-Fischer solution loses its capability...
02 July 2024 5,044 3 View
Greetings and Regards I have a question about bibliometric articles about public health or nursing. If any of the researchers have experience or knowledge in this matter, please tell me. Thank You
15 June 2024 2,795 1 View
Hi everyone Is there any guideline for method validation for cosmetic? I wanna know about criteria for that.
31 May 2024 1,990 2 View
I am working on a project to detect genetic mutations in lung cancer using machine learning algorithms and I am new to genetics and medical sciences. Can I use RNA as input to the algorithm for...
13 May 2024 5,726 6 View
Generally when they want to use hydrogel for MTT assay, firstly they fix hydrogel with glutaraldehyde then use for MTT assay. so, now, i have self healing hydrogel and its have crosslinker in its...
11 May 2024 6,310 1 View
Hi Have a nice day I want to determination PAAs and PAHs in tatoo. Would you present and introduce methods to this?
02 April 2024 6,772 0 View
"Hi, I gasify the polyethylene terephthalate granules and mold them using a hot press at a temperature of 260 degrees Celsius to prepare a bulk sample. However, after molding with the hot press,...
31 March 2024 8,668 0 View
I have a dataset from lung cancer images with 163 samples (2D images). I use the fine-tuning of deep learning algorithms to classify samples, but the validation loss did not decrease. I augmented...
21 March 2024 8,344 6 View
Hello, I want to dissolve polyphthalamide in a solvent. I tested different solvents, formic acid, toluene, DMSO, alcohol-based solvents, etc. under high temperature and time, but it did not...
12 March 2024 3,030 1 View
Hi. I need to remove all microplastics of a wastewater sample without changing any physicochemical properties of the sample. Can filtration by Whatman filters (for example Whatman 589/3 with...
08 March 2024 8,722 3 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View