Hi,
would anyone have recommendations for a set of plasmids for endogenous C-terminal protein tagging in S. cerevisiae (i.e. plasmids as template for the PCR product to transform). I`m looking for any common tags with common resistance or auxotrophic markers, e.g. GFP, TAP, HA, FLAG, Myc with KanR, HphR, Leu/Ura/His marker? In my last lab we used the Janke, 2004 set (pYM..), but apparently there are points in the MTA with EMBL that some US universities are unwilling to accept.
We have another set here, but I never get the same number of colonies or generally clean colonies over no background. I`m not sure what`s going on, but I suspect the selection markers aren`t expressed well.
Any help would be appreciated! Thanks,
Michael
Hi, You can use pFA6a-GFP(S65T)-kanMX6 plasmid as a template.
Here is the paper
Article Heterologous modules for efficient and versatile PCR-based g...
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