It won't solidify properly and I don't know why. I'm using dry XLD agar formula provided by BTL (Poland)
Szymon,
For XLD the best procedure is to prepare maximum 500mL, waste the powder, then add hot water slowly and shake it vigorously, when is totally disolved, put it in a hot water bath (80-90º C) shake it every 5 min, when the agar is clearly take it out and serve in plates.
hope it works,
Xylose lysine deoxycholate agar is a selective growth medium used in the isolation of Salmonella and Shigella species from clinical samples and from food. (XLD) It was developed by Taylor for the differentiation, isolation, and identification of enteric pathogens, and to support the growth of more fastidious enteric organisms. XLD Agar was especially designed to allow the growth of Shigella species, and is a proven medium for the isolation of this organism. It has also been found to be an excellent medium for isolating Salmonella species as well.
Ingredients per liter of deionized water are as following
Lactose 7.5gm
Sucrose 7.5gm
Sodium Thiosulfate 6.8gm
L-Lysine 5.0gm
Sodium Chloride 5.0gm
Xylose 3.75gm
Yeast Extract 3.0gm
Sodium Deoxycholate 2.5gm
Ferric Ammonium Citrate 0.8gm
Phenol Red 0.08gm
Agar 15.0gm
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